OLS : Record

RECORD NUMBER: 15 OF 143

Main Title Applications for DNA Probes in Biodegradation Research.
Author Sayler, G. S.; Jain, R. K.; Ogram, A.; Pettigrew, C. A.; Houston, L.;
CORP Author Tennessee Univ., Knoxville.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1986
Report Number EPA/600/D-86/249; EPA-R-812488;
Stock Number PB87-145322
Subjects Deoxyribonucleic acids; Biodeterioration; Toxicology; Contaminants; Naphthalene; Ecology; Industrial waste treatment; Activated sludge process; Ground water; Hybridization; DNA; Biodegradation; Environmental pollutants; Refuse disposal; Industrial waste
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB87-145322 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 36p
Abstract The use of DNA:DNA hybridization technology in biodegradation studies is investigated. The rate constants for sediments exposed to synthetic oils could be calculated from the NAH(1+) genotypes and this approach would be useful in predicting the kinetics of aromatic hydrocarbon degradation. Gene probes prepared from NAH7 plasmid were also used to monitor and enumerate the naphthalene-degrading populations in a continuous mixed culture bioreactor and this analysis demonstrated at least one order of magnitude difference in the naphthalene-degrading population over the conventional plate analysis. It was also shown that using pSS50 (a chlorobiphenyl mineralizing plasmid) as probe DNA, other polychlorinated biphenyls degrading organisms can be identified from the environment. Further, the maintenance and stability of a genetically modified Pseudomonas putida (carrying plasmids TOL and RK2) over an 8-week period in chemically contaminated groundwater aquifer material was established.
Supplementary Notes Sponsored by Environmental Research Lab., Gulf Breeze, FL.
PUB Date Free Form Dec 86
Category Codes 57K; 57B
NTIS Prices PC A03/MF A01
Primary Description 600/04
Document Type NT
Control Number 709318361
Cataloging Source NTIS/MT
Origin NTIS
Type CAT