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Main Title Separation and Detection of a Benzo(a)pyrene Deoxyguanosy-5-monophosphate Adduct by Capillary Zone Electrophoresis.
Author Jackim, E.; Norwood, C.;
CORP Author Environmental Research Lab., Narragansett, RI.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/075 ;ERLN-1098;
Stock Number PB90-245523
Subjects Electrophoresis; Thin layer chromatography; Ultraviolet spectroscopy; Nucleotides; Reprints; DNA adducts; Benzo(a)pyrene; High pressure liquid chromatography; Phosphorus radioisotopes
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB90-245523 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/03/1990
Collation 4p
Abstract The detection of DNA adducts from environmentally exposed organisms is difficult because of their low concentration (one adduct in 10 to the 7th power - 10 to the 9th power normal nucleotides). The most feasible way of detecting these adducts has been by radioactive P(sup 32) post labeling and separation by multidimensional thin layer chromatography, or more recently by monitoring the radioactivity after HPLC separation. The procedure lacks precision, is difficult to perform, and poses health risks because of the high specific P(sup 32) radioactivity required. Capillary Zone Electrophoresis (CZE) with fluorescent or indirect fluorescent detection offers the potential of comparable detection sensitivity with much greater resolution if the adducts can be separated. Here evidence is present that a benzo(a)pyrene guanosine monophosphate adduct can be separated from normal nucleotides and detected by CZE using a UV absorption detector. (Copyright (c) Dr. Alfred Huethig Publishers.)
Supplementary Notes Pub. in Jnl. of High Resolution Chrotography 13, p195-196 1990.
NTIS Title Notes Journal article.
Title Annotations Reprint: Separation and Detection of a Benzo(a)pyrene Deoxyguanosy-5-monophosphate Adduct by Capillary Zone Electrophoresis.
Category Codes 99A
NTIS Prices PC A01/MF A01
Primary Description 600/05
Document Type NT
Control Number 028529156
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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