OLS : Record

RECORD NUMBER: 34 OF 44

Main Title Use of Immunofluorescence and Phase-Contrast Microscopy for Detection and Identification of 'Giardia' Cysts in Water Samples.
Author Sauch, J. F.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/365;
Stock Number PB86-177771
Subjects Cysts; Disease vectors; Surface waters; Water pollution; Monitoring; Reprints; Giardiasis; Fluorescent antibody technique; Waterborne diseases
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB86-177771 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 6p
Abstract A method was developed in which indirect immunofluorescence and phase-contrast microscopy are used for rapid detection and identification of Giardia cysts in raw and finished water supplies. When anti-Giardia cyst antiserum and fluorescein conjugate were applied to known Giardia cysts on membrane filters, the cysts fluoresced bright green when they were illuminated by UV light. This procedure permitted individual cysts to be quickly located even in samples heavily contaminated with other microorganisms and debris. The identity of presumptive Giardia cysts located in this way could then be confirmed by observing characteristic internal morphological features with phase-contrast microscopy. With this method, Giardia cysts were detected and their identities were confirmed in samples taken from raw and finished surface water supplies during several recent outbreaks.
Supplementary Notes Pub. in Applied and Environmental Microbiology, v50 n6 p1434-1438 Dec 85.
NTIS Title Notes Journal article.
Title Annotations Reprint: Use of Immunofluorescence and Phase-Contrast Microscopy for Detection and Identification of 'Giardia' Cysts in Water Samples.
PUB Date Free Form 1985
Category Codes 6E; 44G; 68D; 57N
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Control Number 614223802
Cataloging Source NTIS/MT
Origin NTIS
Type CAT