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RECORD NUMBER: 31 OF 45

Main Title Production of Viable 'Giardia' Cysts in vitro: Determination by Fluorogenic Dye Staining, Excystation, and Animal Infectivity in the Mouse and Mongolian Gerbil (Journal Version).
Author Schupp, D. G.; Januschka, M. M.; Sherlock, L. A. F.; Stibbs, H. H.; Meyer, E. A.;
CORP Author Minnesota Univ., Minneapolis. ;Oregon Univ. Health Sciences Center, Portland. Dept. of Microbiology and Immunology. ;Washington Univ., Seattle. School of Public Health and Community Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/116;
Stock Number PB89-109359
Subjects Giardiasis; Parasitic intestinal diseases; Protozoa; Giardia; Cultured cells; Cysts; Immunohistochemistry; Morphology; Electron microscopy; Laboratory animals; Life cycle; Infectivity; Gerbils; Reprints; Trophozoites
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB89-109359 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/14/1989
Collation 13p
Abstract The purpose of the research was to document the formation of viable Giardia cysts in vitro. Viability staining, using fluorogenic dyes that required metabolic conversion for detection, and immunocytochemistry at the light microscopic level, provided information on viability as well as the identification of antigens that were similar to those found with in vitro formed cysts. Analysis of cysts formed in vivo and in vitro showed similar morphological appearances by both light and electron microscopy. Cysts formed in vitro were capable of establishing infections in both the mouse and gerbil models for giardiasis. Trophozoites recovered from mice experimentally infected with cysts produced in vitro could be maintained in culture and induced a second time to form cysts in vitro. The model, for the production of viable Giardia cysts in vitro, should facilitate research on controlling the complete life-cycle of Giardia outside an animal host. (Copyright (c) 1988 by the American Gastroenterological Association.)
Supplementary Notes Pub. in Gastroenterology, v95 n1 p1-10 Jul 88. Prepared in cooperation with Oregon Univ. Health Sciences Center, Portland. Dept. of Microbiology and Immunology, and Washington Univ., Seattle. School of Public Health and Community Medicine. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Production of Viable 'Giardia' Cysts in vitro: Determination by Fluorogenic Dye Staining, Excystation, and Animal Infectivity in the Mouse and Mongolian Gerbil (Journal Version).
Category Codes 57N; 57K
NTIS Prices PC A03/MF A01
Primary Description 600/11
Document Type NT
Control Number 834423984
Cataloging Source NTIS/MT
Origin NTIS
Type CAT