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RECORD NUMBER: 24 OF 44

Main Title Giardia lamblia: Stimulation of Growth by Human Intestinal Mucus and Epithelial Cells in Serumfree Medium (Journal Version).
Author Gault, M. J.; Gillin, F. D.; Zenian, A. J.;
CORP Author California Univ. Medical Center, San Diego.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1987
Year Published 1987
Report Number EPA-R-811950; EPA/600/J-87/393;
Stock Number PB89-106017
Subjects Culture media; Parasitic intestinal diseases; Protozoa; Giardiasis; Mucus; Lipids; Growth; Cultured cells; Epithelium; Small intestine; Reprints; Giardia lamblia; Trophozoites
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB89-106017 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/14/1989
Collation 11p
Abstract Giardia lamblia trophozoites specifically colonize the upper human small intestine which is normally serum-free, but grow in vitro only in medium supplemented with serum or serum fractions. Recently, biliary lipids were shown to support the growth of G. lamblia without serum. Now, it is reported that human duodenaljejunal mucus stimulates growth of Giardia in medium with biliary lipids. Stimulation by mucus was enhanced by inclusion of chymotrypsin or crude pancreatic proteases. Coculture of trophozoites with human intestinal epithelial cells also promoted growth, especially in the presence of mucus and/or biliary lipids. With biliary lipids alone, the mean increase in cell number was 3.2 fold and in the presence of mucus 8 fold (p < 0.01) in 24 serial subcultures. The demonstration that human intestinal mucus and epithelial cells promote serum-free growth of G. lamblia may help to explain specific colonization of the small intestine by G. lamblia.
Supplementary Notes Pub. in Experimental Parasitology, v64 p29-37 Aug 87. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Giardia lamblia: Stimulation of Growth by Human Intestinal Mucus and Epithelial Cells in Serumfree Medium (Journal Version).
Category Codes 57N; 57K
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Control Number 833027628
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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