OLS : Record

RECORD NUMBER: 4 OF 48

Main Title

Comparison of static-replacement and flow-through bioassays using duckweed 'Lemna gibba' G-3

Author

CORP Author

Breedlove Associates, Inc., Gainesville, FL.;Environmental Protection Agency, Washington, DC. Office of Pesticides and Toxic Substances.

Publisher

U.S. Environmental Protection Agency, Office of Pesticides and Toxic Substances,

Place Published

Washington, D.C. :

Year Published

1981

Report Number

EPA 560/6-81-003; EPA-68-01-5776

Stock Number

PB81-187650

OCLC Number

18439092

Subjects

Toxicology; Bioassay; Comparison; Mortality; Reproduction(Biology); Data processing; Cost analysis; Cresols; Chlorine organic compounds; Ethylene glycol; Duckweed; Lemna gibba; Toxic substances; Water pollution effects(Animals); Copper sulfate; TCP 2-4-6 herbicide; Phenol/trichloro; Phthalic acid/bis(ethylhexyl-ester)

Subject Added Ent

Lemna-Analysis; Lemnaceae

Internet Access

Description	Access URL
	http://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=9100AP3H.txt

Holdings

Library		Call Number	Additional Info	Location	Date Modified
EJED		EPA 560/6-81-003		OCSPP Chemical Library/Washington,DC	09/22/2000
NTIS		PB81-187650	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.	NTIS	01/01/1988

Collation

xi, 92 p. ; ill. : 28 cm.

Abstract

Static-replacement and flow-through tests were conducted using CuSO4.5H2O, 2,4,6-trichlorophenol, and o-cresol to determine if they gave similar LC50's and EC50's for duckweed, Lemna gibba G-3. Static-replacement tests also were conducted using ethylene glycol and di(2-ethylhexyl) phthalate. Mortality, reproduction, dry weight, and root length were used to measure effect levels of the toxicants. LC50's and EC50's were calculated using quadratic regression with log transformation of the independent variable (concentration) and with several different transformations for the dependent variables. ANOVA's were used to test for differences between the two types of tests, tests within types, and replicates within tests. A procedure also was provided for estimating the number of tests and replicates necessary to obtain confidence limits within a given percentage of the mean. Mortality and reproduction produced the best results. The highest variation occurred among tests, regardless of type, and the smallest variation was generally within tests (i.e. among replicates). Therefore, the best allocation of resources would be to replicate static-replacement tests in time, using four replicates per test.

Notes

"Project Officer, David A. Mauriello, Office of Pesticides and Toxic Substances." "January, 1981." "EPA 560/6-81-003."

Alternate Title

Duckweed 'Lemna gibba' G-3

PUB Date Free Form

1981.

NTIS Prices

PC A06/MF A01

BIB Level

OCLC Time Stamp

20000912083302

Cataloging Source

OCLC/T

Language

eng

Origin

OCLC

Type

MERGE

OCLC Rec Leader

00895cam 2200253Ia 45010

OLS : Record

RECORD NUMBER: 4 OF 48

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