||Application of Tradescantia Micronucleus Assay for In situ Evaluation of Potential Genetic Hazards from Exposure to Chemicals at a Wood-Preserving Site.
Sandhu, S. S.;
Gill, B. S.;
Casto, B. C.;
Rice, J. W.;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||In situ evaluation of biological hazards from wood preserving chemicals at a hazardous waste site using the Tradescantia micronucleus assay (Trad-MCN) is reported in the study. The chemical analysis of soil samples from the site showed high contamination with arsenic (1,292 mg/kg), chromium (1,444 mg/kg) and copper (924 mg/kg) on the surface of the drip area where wood was allowed to dry after the treatment. Exposure of Tradescantia plants grown within or adjacent to the drip area showed significantly (P=0.05) higher frequencies of micronuclei when compared to plants grown in nearby control plots. After remediation, the levels of arsenic, chromium, and copper were substantially reduced and concomitantly no genotoxic activity was observed in plants grown on-site or in plants exposed in the laboratory to a slurry of soil samples from the site. The results from the pilot study show that simple plant assays such as Trad-MCN can be used as a sentinel for environmental contamination and to assess the efficacy of clean-up efforts.
||Pub. in Hazardous Waste and Hazardous Materials, v8 n3 p257-262 Sep 91. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Application of Tradescantia Micronucleus Assay for In situ Evaluation of Potential Genetic Hazards from Exposure to Chemicals at a Wood-Preserving Site.
||68C; 68G; 57H; 57C
||PC A02/MF A01