OLS : Record

RECORD NUMBER: 13 OF 37

Main Title

Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Membrane-Thermotolerant Escherichia coli Agar (mTEC), March 2010.

CORP Author

Environmental Protection Agency, Washington, DC. Office of Water.

Year Published

2010

Report Number

EPA/821/R-10/002

Stock Number

PB2010-114246

Subjects

Escherichia coli; Membrane filtration; Water sampling; Bacteria; Agar; Enumeration; Epidemiology; Microorganisms; Criteria; Water quality; Marine environment; Health effects; Gastroenteristis; Feces; Water pollution effects; Surface waters; Counting methods; Calibration; Growth(Biology); Contamination; Quality control; Pollution prevention; Laboratories; Test methods

Holdings

Library		Call Number	Additional Info	Location	Date Modified
NTIS		PB2010-114246	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.	NTIS	01/21/2011

Collation

46p

Abstract

The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.4). A water sample is filtered through the membrane which retains the bacteria. After filtration, the membrane is placed on a selective and differential medium, mTEC, incubated at 35 degrees C + or = 0.5 degrees C for 2 + or = 0.5 hours to resuscitate injured or stressed bacteria, and then incubated at 44.5 degrees C + or = 0.2 degrees C for 22 + or = 2 hours. Following incubation, the filter is transferred to a filter pad saturated with urea substrate. After 15 minutes, yellow, yellow-green, or yellow-brown colonies are counted with the aid of a fluorescent lamp and a magnifying lens.

Supplementary Notes

OLS : Record

RECORD NUMBER: 13 OF 37

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