OLS : Record

RECORD NUMBER: 17 OF 360

Main Title Assessing the Effect of Colony Counting Methods and Genetic Drift on Ames Bioassay Results.
Author Claxton, L. D.; Toney, S.; Perry, E.; King, L.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC.
Year Published 1984
Report Number EPA/600/J-84/083;
Stock Number PB84-244151
Subjects Counting; Bacteria; Salmonella; Bioassay; Assessments; Reprints; Ames test; Colony counting
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB84-244151 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 15p
Abstract Accuracy of automatic colony counters and variation among subcultures of the Salmonella tester strains are two factors that cause variation within the Ames bioassay. This paper examines these potential sources of variation. In contrast to results seen by other researchers, this genetic drift study demonstrated that except for TA100 the subcultures of tester strains from four research laboratories are very stable in biochemical characteristics measured using Analytical Profile Index (API) strips. This study also documented the levels of accuracy for hand- and automatic-counting techniques, and provides a simple method for generating acetate calibration transparencies for use with most colony counters.
Supplementary Notes Prepared in cooperation with Northrop Services, Inc., Research Triangle Park, NC.
Availability Notes Pub. in Environmental Mutagenesis 6, p331-342 1984.
NTIS Title Notes Journal article.
Title Annotations Reprint: Assessing the Effect of Colony Counting Methods and Genetic Drift on Ames Bioassay Results.
PUB Date Free Form c1984
Category Codes 6M; 57K
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Control Number 435625655
Cataloging Source NTIS/MT
Origin NTIS
Type CAT