OLS : Record

RECORD NUMBER: 15 OF 27

Main Title

Induction of Activation Antigens on Human Natural Killer Cells Mediated Through the Fc-Gamma Receptor.

Author

Harris, D. T.; Travis, W. W.; Koren., H. S.;

CORP Author

Health Effects Research Lab., Research Triangle Park, NC. Clinical Research Branch. ;Duke Univ. Medical Center, Durham, NC. ;North Carolina Univ. at Chapel Hill.

Publisher

c1989

Year Published

1989

Report Number

EPA/600/J-89/467;

Stock Number

PB91-116004

Subjects

Antigens; Deoxyribonucleic acids; Reprints; Natural killer cells; Fluorescent antibody technic; Cross-linking reagents; Interleukin-2; Monoclonal antibodies; Endogenous substances receptors

Holdings

Library		Call Number	Additional Info	Location	Date Modified
NTIS		PB91-116004	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.	NTIS	03/04/1991

Collation

Abstract

NK cells, defined here as lymphocytes bearing the CD16 Ag found on the NK cell Fc-gamma receptor (FcR), are known to enter a proliferative and activated state in response to stimulation with IL-2 as assessed by clonal expansion, short-term DNA synthesis, and de novo expression of lymphocyte-associated activation Ag. We have found that the FcR of NK cells acts as a signaling pathway through which IL-2-dependent activation may be greatly enhanced, allowing for more rapid induction of activation Ag and recruitment of an increased percentage of cells expressing surface markers of cellular activation. FcR-interactive agents, such as solid phase immobilized immune complexes or cross-linked CD16-specific mAb, work synergistically with rIL-2 to elicit a rapid expression of IL2R and transferrin receptors on greater than 5% of purified NK cells as early as day 3 of culture. IL-2 or FcR-interactive stimuli alone were weak or ineffective stimulators by comparison. In contrast to the induction of de novo activation Ag, DNA synthesis was elicited by IL-2 alone, but was not substantially or consistently enhanced by the subsequent addition of FcR-interactive stimuli. (Copyright (c) 1989 by the American Association of Immunologists).

Supplementary Notes

Pub. in Jnl. of Immunology, v143 n7 p2401-2406 Oct 89. Prepared in cooperation with Duke Univ. Medical Center, Durham, NC., and North Carolina Univ. at Chapel Hill.

NTIS Title Notes

Journal article.

Title Annotations

Reprint: Induction of Activation Antigens on Human Natural Killer Cells Mediated Through the Fc-Gamma Receptor.

Category Codes

57J; 57B

NTIS Prices

PC A02/MF A01

Primary Description

600/10

Document Type

Control Number

036522940

Cataloging Source

NTIS/MT

Origin

NTIS

Type

CAT

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RECORD NUMBER: 15 OF 27

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