||Identification of Organic N-Chloramines In vitro in Stomach Fluid from the Rat After Chlorination.
Scully, F. E.;
Mazina, K. E.;
Ringhand, H. P.;
Chess, E. K.;
Campbell., J. A.;
||Old Dominion Univ., Norfolk, VA. Dept. of Chemical Sciences. ;Battelle Pacific Northwest Labs., Richland, WA. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Health Effects Research Lab., Cincinnati, OH.
In vitro analysis;
High performance liquid chromatography;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||When (36)Cl-N-cholorpiperidine (NCP) (300 mg/L as Cl2) was mixed with stomach fluid from laboratory rats (fasted either 24 or 48 hr) and incubated in the dark for 30 min at 37 C and at higher pH values, approximately one-third was reduced to chloride and between 7% and 63% of the radiolabel was found to chromatograph in a manner distinctly different from (36)Cl-chloride of (36)Cl-NCP. The new fraction is referred to as the (36) Cl-chloroorganic fraction. The remainder of the radiolabel was associated with (36)Cl-NCP. Varying concentrations of (36)Cl-NCP (3-200 mg/L as Cl2) have been incubated for 30 min at 37 C with stomach fluid at varying pH on the product distribution. At low pH values a threshold concentration of approximately 100 mg/L active chlorine was measured below which all chloramine was reduced to (36)Cl-chloride. As the pH and concentration of (36)Cl-NCP were increased, the percentage of chloramine reduced to chloride decreased. Using concentrations between 110 and 120 mg/L (Cl2) 7% and 4% of the label was associated with the (36)Cl-chloroorganic fraction at pH 7.1 and at pH 2.2, respectively.
||Pub. in Chemical Research in Toxicology, v3 n4 p301-306 Jul 90. Presented at International Symposium on Health Effects of Drinking Water Disinfectants and Disinfection By-Products (2nd), Cincinnati, OH., August 27-29, 1985. Prepared in cooperation with Battelle Pacific Northwest Labs., Richland, WA., and North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.
|NTIS Title Notes
||Reprint: Identification of Organic N-Chloramines In vitro in Stomach Fluid from the Rat After Chlorination.
||PC A02/MF A01