Record Display for the EPA National Library Catalog
RECORD NUMBER: 50 OF 98| Main Title | Microwave Irradiation for Rapid Killing and Fixing of Plant Tissue. | |||||||||||
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| Author | Walsh, G. E. ; Bohannon, P. M. ; Wessinger-Duvall, P. B. ; | |||||||||||
| CORP Author | Environmental Research Lab., Gulf Breeze, FL. | |||||||||||
| Publisher | 1989 | |||||||||||
| Year Published | 1989 | |||||||||||
| Report Number | EPA/600/J-89/204; | |||||||||||
| Stock Number | PB90-147802 | |||||||||||
| Additional Subjects | Microwaves ; Plant tissues ; Irradiation ; Histology ; Exposure ; Cell nucleus ; Survival ; Destruction ; Chloroplasts ; Reprints ; Fixatives | |||||||||||
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| Collation | 5p | |||||||||||
| Abstract | Irradiation by microwaves allows for rapid killing and fixing of plant tissue, with excellent cellular integrity for histological examination. One or two exposures to microwaves for three seconds in formalin/acetic acid/alcohol gave good preservation of nuclei, chloroplasts, and other plant structures. The microwave method offers a considerable saving of time over traditional methods for killing and fixing plant tissue. |