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Grantee Research Project Results

Publications Details for:

A High Throughput Zebrafish Embryo Gene Expression System for Screening Endocrine Disrupting Chemicals
Grant Number R831301
RFA: Development of High-Throughput Screening Approaches for Prioritizing Chemicals for the Endocrine Disruptors Screening Program (2003)


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Abstract (8)
Journal Article (7)
Presentation (39)
Reference Type Citation Progress Report Year Document Sources
Abstract Burnam L, Novillo A, Callard GV. Development of an endocrine disrupter screen based on gene expression in zebrafish embryos. The Journal of Experimental Zoology Part A:Comparative and Experimental Biology 2006;305A(2):114.
R831301 (Final)
Abstract Callard GV. Regulation and dysregulation of aromatase and estrogen receptor genes in fishes. The Journal of Experimental Zoology Part A: Comparative and Experimental Biology 2006;305A(2).
R831301 (2004)
R831301 (Final)
Abstract Emmith N, Novillo A, Callard G. Multispecies comparison of estrogen receptors in teleosts. The Journal of Experimental Zoology Part A:Comparative and Experimental Biology 2006;305A(2).
R831301 (Final)
Abstract Greytak SR, Callard GV. Cloning of three estrogen receptor (ER) forms in killifish (Fundulus heteroclitus):differential expression and regulation in fish from polluted (New Bedford Harbor, NBH) and unpolluted environments. The Journal of Experimental Zoology Part A:Comparative and Experimental Biology 2006;305A(2).
R831301 (Final)
Abstract McCurley A, Novillo A, Callard GV. Estrogen signaling during neuroregeneration in zebrafish. The Journal of Experimental Zoology Part A:Comparative and Experimental Biology 2006;305A(2).
R831301 (Final)
Abstract Novillo A, Kishida M, Toledo M, Callard IP, Callard GV. Environmental chemicals induce a novel splice variant of the brain form of cytochrome P450 aromatase in zebrafish embryos. The Journal of Experimental Zoology Part A:Comparative and Experimental Biology 2006;305A(2).
R831301 (2004)
R831301 (Final)
Abstract Novillo A, Wood B, Ciaccia L, Callard G. Multispecies comparison of cyp19 (cytochrome P450 aromatase) genes in teleosts. The Journal of Experimental Zoology Part A: Comparative and Experimental Biology 2006;305A(2).
R831301 (Final)
Abstract Sawyer S, Callard GV. Cloning and characterization of orphan nuclear receptors Nurr1, Nur77 and Nor1 in zebrafish: developmental programming, tissue specificity and estrogen regulation. The Journal of Experimental Zoology Part A: Comparative and Experimental Biology 2006;305A(2).
R831301 (Final)
Journal Article Burnett KG, Bain LJ, Baldwin WS, Callard GV, Cohen S, Di Giulio RT, Evans DH, Gomez-Chiarri M, Hahn ME, Hoover CA, Karchner SI, Katoh F, MacLatchy DL, Marshall WS, Meyer JN, Nacci DE, Oleksiak MF, Rees BB, Singer TD, Stegeman JJ, Towle DW, Van Veld PA, Vogelbein WK, Whitehead A, Winn RN, Crawford DL. Fundulus as the premier teleost model in environmental biology: opportunities for new insights using genomics. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 2007;2(4):257-286.
abstract available     full text available
R831301 (Final)
Journal Article Greytak SR, Champlin D, Callard GV. Isolation and characterization of two cytochrome P450 aromatase forms in killifish (Fundulus heteroclitus): differential expression in fish from polluted and unpolluted environments. Aquatic Toxicology 2005;71(4):371-389.
abstract available     full text available
R831301 (2006)
R831301 (Final)
Journal Article Greytak SR, Callard GV. Cloning of three estrogen receptors (ER) from killifish (Fundulus heteroclitus): differences in populations from polluted and reference environments. General and Comparative Endocrinology 2007;150(1):174-188.
abstract available     full text available
R831301 (2006)
R831301 (Final)
Journal Article Greytak SR, Tarrant AM, Nacci D, Hahn ME, Callard GV. Estrogen responses in killifish (Fundulus heteroclitus) from polluted and unpolluted environments are site-and gene-specific. Aquatic Toxicology 2010;99(2):291-299.
abstract available     full text available
R831301 (Final)
Journal Article McCurley AT, Callard GV. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment. BMC Molecular Biology 2008;9:102.
abstract available     full text available
R831301 (Final)
Journal Article Sawyer SJ, Gerstner KA, Callard GV. Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation. General and Comparative Endocrinology 2006;147(2):108-117.
abstract available     full text available
R831301 (2004)
R831301 (2006)
R831301 (Final)
Journal Article Tarrant AM, Greytak SR, Callard GV, Hahn ME. Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness. Journal of Molecular and Cellular Endocrinology 2006;37(1):105-120.
abstract available     full text available
R831301 (2006)
R831301 (Final)
Presentation Blazquez M, Gonzalez A, Callard GV, Piferrer F. Cloning of vasa in the European sea bass (Dicentrarchus labrax): mRNA expression levels during sex differentiation. Poster presented at the 5th International Symposium on Fish Endocrinology, Castellon, Spain, September 2004.
R831301 (Final)
not available
Presentation Burnam L, Novillo A, Callard GV. Development of an endocrine disrupter screen based on gene expression in zebrafish embryos. Poster presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (abstract P14.12, p. 114).
R831301 (2005)
R831301 (Final)
not available
Presentation Burnam L, Novillo A, Callard GV. Development of an endocrine disruptor screen based on gene expression in zebrafish embryos. Poster presented at the Superfund Basic Research Program Annual Meeting, New York, NY, January 2006 (Abstract #44).
R831301 (2006)
R831301 (Final)
not available
Presentation Burnam L, Novillo A, Callard GV. Analysis of gene expression changes in zebrafish embryos as a whole animal screening bioassay for endocrine disrupting chemicals. Poster presented at ENDO 2006, the 88th Annual Meeting of the Endocrine Society, Boston, MA, June 24-27, 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Burnam L. Analysis of gene expression changes in zebrafish embryos as an endocrine disruptor screen. Poster presented at the EPA STAR Fellows Annual Meeting, Washington, DC, September 2006.
R831301 (Final)
not available
Presentation Burnam L, Novillo A, Callard GV. Analysis of gene expression changes in zebrafish embryos as a whole animal screening bioassay for endocrine disrupting chemicals. Poster presented at the Society of Environmental Toxicology and Chemistry Regional Meeting, Portland, ME, June 7-9, 2006.
R831301 (2006)
not available
Presentation Callard GV, Novillo A, Sawyer SJ. A high throughput zebrafish embryo gene expression system for screening endocrine disrupting chemicals. Poster presented at the 2004 U.S. EPA Science Forum, Washington, DC, June 2004.
R831301 (2004)
R831301 (Final)
not available
Presentation Callard GV. Regulation and dysregulation of aromatase and estrogen receptor genes in fishes. Presented at the 5th International Symposium on Fish Endocrinology, Castellon, Spain, September 2004 (state-of-the-art-lecture).
R831301 (2004)
R831301 (Final)
not available
Presentation Callard GV. Regulation and dysregulation of aromatase and estrogen receptor genes in fishes. Presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (Abstract 2.1, p. 115; state-of-the-art lecture).
R831301 (Final)
not available
Presentation Callard GV, Greytak S, Champlin D, Tarrant A, Hahn M. Reproductive and developmental effects and adaptations in killifish (Fundulus heteroclitus) after chronic, multigenerational exposure to estrogenic contaminants at a Superfund site. Poster presented at the U.S. EPA Science Forum, Washington, DC, May 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Callard GV. The synaptocrine hypothesis: history, future perspectives and lessons from fish. Presented at the 11th Biannual Meeting of the International Neurotoxicology Association, Pacific Grove, CA, June 10-15, 2007 (symposium lecture).
R831301 (Final)
not available
Presentation Emmith N, Novillo A, Callard G. Multispecies comparison of estrogen receptors in teleosts. Poster presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (Abstract P11.1, p. 124).
R831301 (2005)
R831301 (Final)
not available
Presentation Greytak GV, Novillo A, Wood E, Callard GV. Fish aromatases: functions, dysfunctions, adaptations and evolution. Aromatase Symposium. Lecture presented at the 7th International Congress of Comparative Physiology and Biochemistry, Salvador-Bahia, Brazil, August 12-16, 2007.
R831301 (Final)
not available
Presentation Greytak SF, Callard GV. Long term multigenerational reproductive effects and adaptations in killifish (Fundulus heteroclitus) from the New Bedford Harbor (NBH) Superfund Site. Poster presented at the Annual Meeting of the Superfund Basic Research Program, Hanover NH, November 2003.
R831301 (Final)
not available
Presentation Greytak SR, Hahn ME, Callard GV. Molecular cloning of aromatases (Arom) and estrogen receptors (ER) in killifish (Fundulus heteroclitus):differences in constitutive and inducible expression in populations from polluted and reference sites. Presented at the 4th Society of Environmental Toxicology and Chemistry (SETAC) World Congress, Portland OR, November 2004.
R831301 (Final)
not available
Presentation Greytak SR, Callard GV. Cloning of three estrogen receptor (ER) forms in killifish (Fundulus heteroclitus):differential expression and regulation in fish from polluted (New Bedford Harbor, NBH) and unpolluted environments. Presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (Abstract 2.4, p. 130).
R831301 (Final)
not available
Presentation Greytak SR, Callard GV. Long term multigenerational reproductive effects and adaptations in killifish (Fundulus heteroclitus) from the New Bedford Harbor (NBH) Superfund Site. Poster presented at the Superfund Basic Research Program Annual Meeting, New York, NY, January 2006, (abstract #32).
R831301 (2006)
R831301 (Final)
not available
Presentation Greytak SR, Callard GV. Cloning of three estrogen receptor (ER) cDNAs in killifish (Fundulus heteroclitus): differences in constitutive and inducible expression in populations from polluted (New Bedford Harbor, NBH) and unpolluted (Scorton Creek, SC) environments. Poster presented at ENDO 2006, the 88th Annual Meeting of the Endocrine Society, Boston, MA, June 24-27, 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation McCurley A, Novillo A, Callard GV. Estrogen signaling during neuroregeneration in zebrafish. Poster presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (abstract P15.7, p. 154).
R831301 (Final)
not available
Presentation McCurley A, Novillo A, Callard GV. Estrogen signaling during neuroregeneration in zebrafish. Poster presented at the Superfund Basic Research Program Annual Meeting, New York, NY, January 2006, (abstract #8).
R831301 (2006)
R831301 (Final)
not available
Presentation McCurley A, Callard GV. Brain estrogen biosynthesis during neuroregeneration in zebrafish. presented at the 11th Biannual Meeting of the International Neurotoxicology Association, Pacific Grove, CA, June 10-15, 2007.
R831301 (Final)
not available
Presentation McCurley M, Novillo A, Callard GV. Brain estrogen biosynthesis and actions during neurogenesis and neuroregeneration in zebrafish. Poster presented at ENDO 2006, the 88th Annual Meeting of the Endocrine Society, Boston, MA, June 24-27, 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Novillo A, Kishida M, Toledo M, Callard IP, Callard GV. Exposure to environmental chemicals induces a novel splice variant of the brain form of cytochrome P450 aromatase in zebrafish embryos. Poster presented at the Annual Meeting of the Superfund Basic Research Program, Hanover NH, November 2003.
R831301 (2004)
R831301 (Final)
not available
Presentation Novillo A, Kishida M, Toledo M, Callard IP, Callard GV. Environmental chemicals induce a novel splice variant of the brain form of cytochrome P450 aromatase in zebrafish embryos. Poster presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (Abstract P14.16, p. 163).
R831301 (Final)
not available
Presentation Novillo A, Wood B, Ciaccia L, Callard G. Multispecies comparison of cyp19 (cytochrome P450 aromatase) genes in teleosts. Poster presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (Abstract P11.3, p. 163).
R831301 (2005)
R831301 (Final)
not available
Presentation Novillo A, Kishida M, Toledo M, Callard IP, Callard GV. Environmental chemicals induce a novel splice variant of the brain form of cytochrome P450 aromatase in zebrafish embryos. Poster presented at ENDO 2006, the 88th Annual Meeting of the Endocrine Society, Boston, MA, June 24-27, 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Novillo A, Kishida M, Toledo M, Callard IP, Callard GV. Exposure to environmental chemicals induces a novel splice variant of the brain form of cytochrome P450 aromatase in zebrafish embryos. Poster presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 22-27, 2005.
R831301 (2005)
not available
Presentation Sawyer SJ, Callard GV. Characterization of the Nurr/Nor nuclear receptor family in zebrafish: role in mediating neurotoxicity. Poster presented at the Superfund Basic Research Program Annual Meeting, Hanover, NH, November 2003.
R831301 (2004)
not available
Presentation Sawyer SJ, Callard GV. Cloning and characterization of zebrafish Nurr1: a marker of neurotoxicity. Poster presented at the Annual Meeting of the Superfund Basic Research Program, Hanover NH, November 2003.
R831301 (Final)
not available
Presentation Sawyer SJ, Novillo A, Burnam LC, McCurley A, Callard GV. A high throughput zebrafish embryo gene expression system for screening endocrine disrupting chemicals. Presented at the EPA STAR Computational Toxicology Research Symposium, Research Triangle Park, NC, July 2005.
R831301 (2005)
R831301 (2006)
R831301 (Final)
not available
Presentation Sawyer SJ, Callard GV. Orphan nuclear receptors Nurr1, Nur77 and Nor1: evolutionary conservation of gene structure, function, and regulation in vertebrates. Poster presented at ENDO 2006, the 88th Annual Meeting of the Endocrine Society, Boston, MA, June 24-27, 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Sawyer SJ, Callard GV. Orphan nuclear receptors Nurr1, Nur77 and Nor1 in zebrafish: sequence analysis, developmental programming, tissue specificity and regulation. Poster to be presented at the Nuclear Receptors Keystone Symposium, Banff, Canada, March 2006
R831301 (2005)
not available
Presentation Sawyer S, Callard GV. Cloning and characterization of orphan nuclear receptors Nurr1, Nur77 and Nor1 in zebrafish: gene specific developmental progamming, tissue specificity and estrogen regulation. Poster presented at ENDO 2005, the 87th Annual Meeting of the Endocrine Society, San Diego, CA, June 4-7, 2005.
R831301 (Final)
not available
Presentation Sawyer S, Greytak SR, Engel KB, Novillo A, Callard GV. Endocrine disruption: lessons from laboratory and field studies using fish models. Poster presented at ENDO 2005, the 87th Annual Meeting of the Endocrine Society, San Diego, CA, June 4-7, 2005.
R831301 (2005)
R831301 (Final)
not available
Presentation Sawyer S, Callard GV. Cloning and characterization of orphan nuclear receptors Nurr1, Nur77 and Nor1 in zebrafish:developmental programming, tissue specificity and estrogen regulation. Presented at the 15th International Congress of Comparative Endocrinology, Boston, MA, May 2005 (Abstract 10.7, p. 173).
R831301 (2005)
R831301 (Final)
not available
Presentation Sawyer S, Callard G. Molecular modeling of the zebrafish Nurr1 (nr4a2) nuclear receptor LBD by homology with the human Nurr1 LBD crystal structure:insights into regulation of an orphan. Poster presented at the Superfund Basic Research Program Annual Meeting, New York, NY, January 2006 (Abstract #16).
R831301 (2006)
R831301 (Final)
not available
Presentation Sawyer S, Callard G. Orphan nuclear receptors Nurr1, Nur77 and Nor1 in zebrafish: sequence analysis, developmental programming, tissue specificity and regulation. Poster presented at the Keystone Symposium on Nuclear Receptors: Orphan Brothers, Banff Canada, March 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Tarrant AM, Greytak SR, Callard GV, Hahn ME. Estrogen receptor-related receptors in the Atlantic killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness. Poster presented at the Society for Integrative & Comparative Biology Annual Meeting, Orlando, FL, January 2006.
R831301 (2006)
R831301 (Final)
not available
Presentation Yershov A, Novillo A, Callard GV. Characterization of functionally and structurally different estrogen receptor splice variants in zebrafish: differential expression in response to environmental estrogens. Poster presented at the Undergraduate Research Opportunities Symposium, Boston University, Boston, MA, June 2006.
R831301 (Final)
not available

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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