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Extramural Research

Evaluating Nanoparticle Interactions with Skin

EPA Grant Number: R831715
Title: Evaluating Nanoparticle Interactions with Skin
Investigators: Monteiro-Riviere, Nancy A. , Riviere, Jim E.
Institution: North Carolina State University
EPA Project Officer: Savage, Nora
Project Period: September 18, 2004 through September 30, 2007
Project Amount: $328,972
RFA: Exploratory Research to Anticipate Future Environmental Issues: Impacts of Manufactured Nanomaterials on Human Health and the Environment (2003)
Research Category: Nanotechnology , Health Effects

Description:

Objective:

The focus of this research is to assess the nature of interaction between manufactured nanoparticles and the skin, including dermal absorption, cutaneous toxicity as well as the ability to distribute to the skin after systemic exposure. These studies will utilize iron oxide nanocrystals, cadmium selenide nanocrystals and carbon fullerene nanoparticles which are representative of the broad spectrum of nanoparticles presently being used by industry. Eight particle types selected from these commercially relevant manufactured nanoparticles will be studied to allow assessment of size, shape and composition on absorption, distribution or toxicity to the skin.

Approach:

All studies will be conducted in three well-characterized in vitro skin models: human skin keratinocyte cell cultures, porcine skin flow-through diffusion cells, and the isolated perfused porcine skin flap (IPPSF). Nanoparticles will be applied topically in three exposure scenarios (neat, water, mineral oil) at two doses to assess potential dermal absorption in the diffusion cell studies and to assess cellular toxicity (light and electron microscopy, viability) and irritation (IL-8 release) in cell culture. Those particles which penetrate skin or cause direct irritation will then be completely characterized in IPPSF studies which have previously been shown to be predictive of in vivo absorption in humans. Similarly, to model nanoparticle uptake into skin after systemic exposure, nanoparticles will be infused into the arterial blood supply of the IPPSF to assess ability to distribute out of the vasculature into the skin. Deposition of particles in epidermal tissue after both infusion and topical exposure will be evaluated using high-resolution electron microscopy.

Expected Results:

Presently, there are minimal data available on the interaction between manufactured nanoparticles and biological tissues. The basic requirement for any risk assessment includes information on hazard (e.g. toxicity) and exposure (e.g. absorption). This proposal focuses on the health effects of nanoparticle interactions with the skin. This integrated research program will generate data on the ability of nanoparticles to be toxic to keratinocytes as well as assess the ability of nanoparticles to either be absorbed into skin after topical exposure, or distribute into skin as would occur after systemic exposure by an alternate route of administration. At the conclusion of the research, the boundaries of a dermal risk assessment for manufactured nanoparticle exposure will be available.

Publications and Presentations:

Publications have been submitted on this project: View all 119 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 17 journal articles for this project

Supplemental Keywords:

nanoparticles, nanotubes, skin absorption, skin toxicity, skin exposure, dermal absorption, toxicologic pathology, in vitro methods, cell culture, IPPSF, health effects, catalysts, cellular toxicity, Health, Scientific Discipline, ENVIRONMENTAL MANAGEMENT, Environmental Chemistry, Health Risk Assessment, Risk Assessments, Biology, Risk Assessment, toxicology, dermal contact, nanotechnology, carbon fullerene, cutaneous toxicity, iron oxide nanocrystals, human exposure, cellular response to nanoparticles, exposure assessment, human health risk, oxidative stress

Progress and Final Reports:
2005 Progress Report
2006 Progress Report

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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