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Extramural Research

Grantee Research Project Results

NCER Grantee Research Project Results

Investigation of Waterborne Microsporidia

EPA Grant Number: U915584
Title: Investigation of Waterborne Microsporidia
Investigators: Mital, Jeffrey T.
Institution: John Jay College of Criminal Justice
EPA Project Officer: Broadway, Virginia
Project Period: September 1, 1999 through August 1, 2001
Project Amount: $60,444
RFA: STAR Graduate Fellowships (1999)
Research Category: Fellowship - Environmental Science/Forensic Science , Academic Fellowships , Ecological Indicators/Assessment/Restoration

Description:

Objective:

The objective of this research project is to develop a simple and efficient method for the routine detection of waterborne microsporidia, and to apply this method to the investigation of microsporidia in surface water.

Approach:

The proposed technique concentrates microsporidia spores by filtration, and detects their presence using polymerase chain reaction (PCR) amplification of the gene that codes for a small subunit of ribosomal RNA in microsporidia. Submicron filters of different material, construction, and pore size are being tested to determine the most efficient filter for capture and recovery of spores. Various methods are being evaluated to determine the most effective means of extracting and isolating amplifiable DNA from within the tough spore. Primers are used to amplify DNA from most species of microsporidia that are infectious to humans. Surface water samples will be tested for the presence of microsporidia using the refined technique. The specific species present then will be determined by a combination of methods. Each species will result in an amplification product of a different length, ranging from 410 to 433 base pairs. The length of the PCR product can be determined, within one base pair, using Perkin Elmer's ABI Prism 310 Genetic Analyzer. Species confirmation will be accomplished by sequencing the PCR product.

Supplemental Keywords:

fellowship, microsporidia, water, polymerase chain reaction, PCR., RFA, Health, Scientific Discipline, PHYSICAL ASPECTS, INTERNATIONAL COOPERATION, Water, Risk Assessments, Environmental Microbiology, Biochemistry, Physical Processes, Biology, Drinking Water, pathogens, microbiological organisms, waterborne disease, detection, exposure, filtration, polymerase chain reaction, human exposure, microbial exposure, microbial risk, dietary ingestion exposures, drinking water contaminants, microsporidia

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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