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Citation
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HERO ID
625047
Reference Type
Journal Article
Title
Bacterial mutagenicity of 2-chloro-1,3-butadiene (chloroprene) caused by decomposition products
Author(s)
Westphal, GA; Blaszkewicz, M; Leutbecher, M; Müller, A; Hallier, E; Bolt, HM
Year
1994
Is Peer Reviewed?
Yes
Journal
Archives of Toxicology
ISSN:
0340-5761
EISSN:
1432-0738
Volume
68
Issue
2
Page Numbers
79-84
Language
English
PMID
8179486
DOI
10.1007/s002040050038
Web of Science Id
WOS:A1994MY31000002
URL
https://search.proquest.com/docview/76481100?accountid=171501
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Abstract
Since the literature on genotoxicity of 2-chloro-1,3-butadiene (chloroprene) is controversial, the mutagenicity of this compound was reinvestigated with respect to its chemical stability. Because of the volatility of chloroprene, Ames tests with S. typhimurium TA 100 were carried out with gas-tight preincubation. Propylene oxide, a volatile direct mutagen, served as a positive control. Benzo[a]pyrene was used as a control for an indirect mutagen. Using this experimental regimen, freshly distilled chloroprene was not mutagenic. However, a mutagenic effect occurred linearly with increasing age of the chloroprene distillates. Aged chloroprene gave the same positive results whether preincubation was gas-tight or not. Analysis by gas chromatography (GC) revealed several decomposition products in aged chloroprene distillates. The direct mutagenicity towards TA 100 correlated with the integrated amounts of four of these substances; these substances always occurred in the same relative ratio. When chloroprene was kept under anaerobic conditions, products occurred with time which were partly different from those obtained under aerobic conditions. The direct mutagenicity of anaerobically aged chloroprene was only weak, but the mutagenic effect was enhanced about two- to threefold by addition of S9 mix. Partial identification of chloroprene decomposition products was done by gas chromatography-mass spectrometry (GC-MS): major byproducts of chloroprene, probably responsible for mutagenic properties of aged chloroprene samples, were cyclic chloroprene dimers.
Keywords
Chloroprene; 2-Chloro-1,3-butadiene; Mutagenicity; Ames test
Tags
IRIS
•
Chloroprene
Cited 2009 Draft
Cited 2010 Final
•
PCBs
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