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5661099 
Journal Article 
High frequency of codon 61 K-ras A-T transversions in lung and Harderian gland neoplasms of B6C3F1 mice exposed to chloroprene (2-chloro-1,3-butadiene) for 2 years, and comparisons with the structurally related chemicals isoprene and 1,3-butadiene 
Sills, RC; Hong, HL; Melnick, RL; Boorman, GA; Devereux, TR 
1999 
Yes 
Carcinogenesis
ISSN: 0143-3334
EISSN: 1460-2180 
20 
4 
657-662 
English 
10223196 
WOS:000079714300020 
Chloroprene is the 2-chloro analog of 1,3-butadiene, a potent carcinogen in laboratory animals. Following 2 years of inhalation exposure to 12.8, 32 or 80 p.p.m. chloroprene, increased incidences of lung and Harderian gland (HG) neoplasms were observed in B6C3F1 mice at all exposure concentrations. The present study was designed to characterize genetic alterations in the K- and H-ras proto-oncogenes in chloroprene-induced lung and HG neoplasms. K-ras mutations were detected in 80% of chloroprene ected in spontaneous lung tumors of B6C3F1 mice and was identified in only 7% of spontaneous HG neoplasms. In lung neoplasms, greater percentages (80 and 71%) of A-T transversions were observed at the lower exposures (12.8 and 32 p.p.m.), respectively, compared with 18% at the high exposure. In HG neoplasms, the percentage of A-T transversions was the same at all exposure concentrations. The chloroprene-induced ras mutation spectra was similar to that seen with isoprene, where th 
1,3 butadiene; carcinogen; chloroprene; isoprene; Ras protein; animal experiment; animal model; article; controlled study; gene mutation; Harder gland; lung cancer; nonhuman; oncogene H ras; oncogene K ras; priority journal