Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
91214
Reference Type
Journal Article
Title
Improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity
Author(s)
Sager, TM; Porter, DW; Robinson, VA; Lindsley, WG; Schwegler-Berry, DE; Castranova, V
Year
2007
Is Peer Reviewed?
1
Journal
Nanotoxicology
ISSN:
1743-5390
EISSN:
1743-5404
Publisher
Taylor & Francis, 11 New Fetter Lane London EC4P 4EE UK, [mailto:info@tandf.co.uk], [URL:http://www.tandf.co.uk]
Volume
1
Issue
2
Page Numbers
118-129
DOI
10.1080/17435390701381596
Web of Science Id
WOS:000260296600005
Abstract
Nanoparticles agglomerate and clump in solution, making it difficult to accurately deliver them for in vivo or in vitro experiments. Thus, experiments were conducted to determine the best method to suspend nanosized particles. Ultrafine and fine carbon black and titanium dioxide were suspended in phosphate buffered saline (PBS), rat and mouse bronchoalveolar lavage fluid (BALF), and PBS containing dipalmitoyl phosphatidylcholine (DPPC) and/or mouse serum albumin. To assess and compare how these various suspension media dispersed the nanoparticles, images were taken using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results of this study show that PBS is not a satisfactory medium to prepare nanoparticle suspensions. However, BALF was an excellent media in which to suspend nanoparticles. The use of PBS containing protein or DPPC alone, in concentrations found in BALF, did not result in satisfactory particle dispersion. However, PBS-containing protein plus DPPC was satisfactory, although less effective than BALF.
Keywords
Nanoparticles; agglomeration; dispersion; titanium dioxide; carbon black; bronchoalveolar lavage fluid
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity