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Citation
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HERO ID
224485
Reference Type
Journal Article
Title
Formation of N-7-(2-carbamoyl-2-hydroxyethyl)guanine in DNA of the mouse and the rat following intraperitoneal administration of [14C]acrylamide
Author(s)
Segerbäck, D; Calleman, CJ; Schroeder, JL; Costa, LG; Faustman, EM
Year
1995
Is Peer Reviewed?
Yes
Journal
Carcinogenesis
ISSN:
0143-3334
EISSN:
1460-2180
Volume
16
Page Numbers
1161-1165
Abstract
Acrylamide is an alkylating agent which reacts very slowly in direct reactions with DNA and is negative in the Ames test, but is carcinogenic in mice and rats. In order to explain the cancer-initiating properties of acrylamide we have studied DNA adduct formation in vitro with a metabolizing system and in vivo in mice and rats following i.p. administration of 14C-labeled acrylamide. A major adduct found in both species was N-7-(2-carbamoyl-2-hydroxy-ethyl)guanine, formed by reaction of the DNA with the epoxide metabolite glycidamide. The levels of this adduct were similar in the different organs of the two rodent species, which supports the notion that glycidamide is relatively evenly distributed among tissues and that the organ-specificity in acrylamide carcinogenesis cannot be explained by a selective accumulation of the DNA-reactive metabolite in target organs.
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