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HERO ID
199838
Reference Type
Technical Report
Title
Test No. 201: Freshwater alga and cyanobacteria, growth inhibition test
Author(s)
Organisation for Economic Co-operation and Development :: OECD
Year
2006
Publisher
OECD Publishing
Location
Paris, France
Book Title
OECD Guidelines for the Testing of Chemicals / Section 2: Effects on Biotic Systems
Number of Pages
26
Language
English
DOI
10.1787/9789264069923-en
URL
http://www.oecdbookshop.org/oecd/display.asp?K=5LMQCR2K7S5H&DS=Test-No.-201-Alga-Growth-Inhibition-Test
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Abstract
The purpose of this test is to determine the effects of a substance on the growth of freshwater microalgae and/or cyanobacteria. Exponentially growing test organisms are exposed to the test substance in batch cultures over a period of normally 72 hours. The system response is the reduction of growth in a series of algal cultures exposed to, at least, five concentrations of a test substance. Three replicates at each test concentration should be used. The response is evaluated as a function of the exposure concentration in comparison with the average growth of control cultures. The cultures are allowed unrestricted exponential growth under nutrient sufficient conditions (two alternative growth media: the OECD and the AAP) and continuous fluorescent illumination. Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. The limit test corresponds to one dose level of 100 mg/L. This study includes: the determination, at least daily, of the algal biomass; the measure of the pH (at the beginning and at the end); microscopic observation. This Test Guideline describes two response variables: average specific growth rate, and yield.
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