Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
194343
Reference Type
Journal Article
Title
Acetone, methyl ethyl ketone, ethyl acetate, acetonitrile and other polar aprotic solvents are strong inducers of aneuploidy in Saccharomyces cerevisiae
Author(s)
Zimmermann, FK; Mayer, VW; Scheel, I; Resnick, MA
Year
1985
Is Peer Reviewed?
1
Journal
Mutation Research
ISSN:
0027-5107
EISSN:
1873-135X
Publisher
Elsevier
Book Title
Mutation Research
Volume
149
Issue
3
Page Numbers
339-351
Language
English
PMID
3887145
DOI
10.1016/0027-5107(85)90150-2
URL
http://linkinghub.elsevier.com/retrieve/pii/0027510785901502
Exit
Abstract
A diploid yeast strain D61.M was used to study induction of mitotic chromosomal malsegregation, mitotic recombination and point mutation. Several ketones (including acetone and methyl ethyl ketone) and some organic acid esters (including the methyl, ethyl and 2-methoxyethyl esters of acetic acid) and acetonitrile strongly induced aneuploidy but not recombination or point mutation. Only diethyl ketone induced low levels of recombination and point mutation in addition to aneuploidy. Related compounds were weak inducers of aneuploidy: methyl n-propyl ketone, the methyl esters of propionic and butyric acid, acetic acid esters of n- and iso-propanol and ethyl propionate. No mutagenicity was found with n-butyl and isoamyl acetate, ethyl formate, acetyl acetone (2,5-dipentanone) and dioxane. Methyl isopropyl ketone induced only some recombination and point mutation but no aneuploidy. Efficient induction was only observed with a treatment protocol in which growing cells were exposed to the chemicals during a growth period of 4 h at 28 degrees C followed by incubation in ice for more than 90 min, usually overnight for 16-17 h. Aneuploid cells could be detected in such cultures during a subsequent incubation at growth temperature if the chemical was still present. Detailed analysis showed that there was a high incidence of multiple events of chromosomal malsegregation. It is proposed that the mutagenic agents act directly on tubulin during growth so that labile microtubules are formed which dissociate in the cold. When cells are brought back to temperatures above the level critical for reassembly of tubulin and allowed to grow, faulty microtubules are formed.
Keywords
Acetates/pharmacology; Acetone/pharmacology; Acetonitriles/pharmacology; Aneuploidy; Butanones/pharmacology; Mitosis/drug effects; Saccharomyces cerevisiae/drug effects; Solvents/pharmacology; Time Factors
Tags
IRIS
•
n-Butanol
Additional Strategies
Source – January 2013 (private)
Additional strategies - 1/2013
Merged reference set - 1/2013
Supporting Studies
Mechanistic and genotoxicity studies
•
1,4-Dioxane - with inhalation update
Final (2013)
External Review Draft (2011)
OPPT REs
•
OPPT_1,4-Dioxane_D. Exposure
Total – title/abstract screening
Supplemental Search
•
OPPT_1,4-Dioxane_F. Human Health
Total – title/abstract screening
On topic
Peer review
Primary source
Cited in IRIS document or IRIS HERO page
On topic - additional tags for titles/abstracts
MOA
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity